Contact an Account ManagerAutomated evaluation of tryptic digest from recombinant human growth hormone using UV spectra and numeric peak information
Summary
Type: HPLC
Application #: 108090
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV spectra between 200 and 350nm in one-second intervals
Conditions: eluent A: 0.1% TFA in water, eluent B: 0.08% TFA in acetonitrile, linear gradient from 0 to 60% B within 120min
flow rate 1ml/min
temperature: 40°C
Authors: Sievert H-J P
Source: J Chromatogr 499 (1990) 221 - 234
Details
Legend: Fig. 1. Tryptic map of r-hGH, Tryptic fragments are identified by FAB-MS and actual amino acid compositions are given in the original paper.